Validating Protein Expression in Exosomes

The analysis of exosome contents of protein is often carried out by high-throughput sequencing or protein quantitative mass spectrometry. Mass spectrometry (MS) is an important tool for finding differentially expressed proteins. In general, the following principles will be followed in the selection of differential proteins:

 

1.Select proteins closely associated with the research or articles.

2.Select proteins with significant differences in quantitative proteome and a small p-value.

3.Select proteins with a relatively high number of unique peptides among differential proteins.

4.Select proteins demonstrating good parallelism and consistent expression trends between groups.

5.Select proteins enriched by GO/KEGG analysis.

 

Based on the principles outlined above, select the target proteins requiring validation. Using Western blot, ELISA, PRM and other methods to verify the protein.

 

Workflow

Services at NextGen Exsome

 

1.Protein in Exosomes Validation Based on Western Blot (WB)

Need additional WB antibodies.

 

Deliverables:

 

1)Images of PVDF membrane development and white light images in TIF format

2)Results of the bands in PPTX format

 

2.Protein in Exosomes Validation Based on Enzyme Linked Immunosorbent Assay (ELISA)

Need additional ELISA kit.

 

Deliverables:

 

1)Report in DOCX format

2)Instrument raw data in PZFX format

 

3.Protein in Exosomes Validation Based on Nano Flow Cytometry (NanoFCM)

Need additional flow cytometry antibodies.

 

Deliverables:

 

1)Sample report in PDF format

2)Instrument raw data in FCS format

 

4.Protein in Exosomes Validation Based on Whole Exome Sequencing (WES)

Need additional WB antibodies.

 

Deliverables:

 

1)Developed picture in PDF format

2)Instrument raw data CBZ format

 

5.Protein in Exosomes Validation Based on Parallel Reaction Monitoring (PRM)

 

Deliverables:

 

1)PVDF film development image and white light image in TIF format

2)Striping results in PPTX format