Exosome Isolation and Purification Service

The isolation of exosomes by magnetic beads refers to the efficient purification and separation of exosomes by binding the exosomes’ surface molecules with magnetic materials such as magnetic beads. This advanced technology equips researchers with a sophisticated approach to procure exosome samples of superior purity and quality. The exosome magnetic bead sorting procedure intricately involves multiple pivotal phases, including the extraction of exosomes, the selection and functionalization of magnetic beads, the binding of exosomes to the beads, the purification of exosomes, and their subsequent detection and analysis. Through these meticulous procedures, researchers can obtain exosome samples of exceptionally high purity for in-depth detection and analysis. Exosome lyophilization involves the removal of water from exosome samples in a cryogenic state through the use of lyophilization technology, aimed at ensuring the long-term preservation and integrity of the samples. This method provides a convenient and efficient solution for researchers to preserve exosomes.

 

Services at NextGen Exsome

 

1.Magnetic Bead Isolation

 

1) Immunomagnetic Separation

Utilizing immunomagnetic beads conjugated with antibodies to capture exosomes expressing specific membrane proteins, followed by separation and recovery using a magnetic rack.

 

Deliverables:

 

Isolated exosomes

 

2)Biotin-Binding Affinity Purification

Employing bead-shaped polyacrylamide resin immobilized with recombinant streptavidin for biotin-binding affinity purification, enabling the sorting and recovery of exosomes expressing specific membrane proteins.

 

Deliverables:

 

Isolated exosomes

 

2.Freeze-Drying

Utilizing mannitol or trehalose as cryoprotectants for lyophilization of exosomes.

 

Deliverables:

 

Exosome lyophilized powder

 

3.Hydrogel Loading

Using medical-grade biodegradable hydrogels for the encapsulation of exosomes.

 

Deliverables:

 

Exosome-loaded hydrogel

 

4.Microneedle Fabrication

Preparing soluble microneedles with lyophilized exosomes.

 

Deliverables:

 

Exosome microneedles