Exosome Functional and Pharmacological Evaluation

In the realm of exosome technology services, the functional evaluation of cellular and animal models necessitates the utilization of these models to assess the biological functions and pharmacological properties of exosomes. This evaluation is pivotal for researchers endeavoring to gain a comprehensive understanding of the biological activities and therapeutic potential of exosomes, both in vivo and in vitro. The service process of functional evaluation using cellular and animal models typically encompasses several key stages: exosome isolation, establishment of relevant cellular or animal models, analysis of the interactions between exosomes and cells or animals, and a comprehensive assessment of the exosomes’ functional and pharmacological profiles. Through these intricate research steps, researchers are optimally equipped to conduct thorough evaluations of the biological functions and pharmacological properties of exosomes, thereby establishing a robust scientific foundation for their clinical applications.

 

Services at NextGen Exsome

1. Cell Proliferation Based Functional Assay

Cell proliferation is assessed using the CCK8 or EDU assay methods.

 

2. Cell Cycle Based Functional Assay

Cell cycle analysis is performed using PI (Propidium Iodide) staining followed by flow cytometry.

 

3.Cell Apoptosis Based Functional Assay

Cell apoptosis is detected using Annexin V/PI dual staining flow cytometry or TUNEL assay methods.

 

4.Cell Invasion Based Functional Assay

Cell invasion is assessed using the transwell assay with a matrigel coating.

 

5.Cell Migration – Scratch Test Based Functional Assay

The cell migration capability is evaluated using a scratch assay performed with a pipette tip.

 

6.Cell Inflammation Based Pharmacological Evaluation

Cell inflammation is induced pharmacologically, followed by the assessment of exosomes and other pharmaceutical compounds for their pro-inflammatory or anti-inflammatory effects.

 

7.Zebrafish Model Based Pharmacological Evaluation

The whitening, repair, anti-inflammatory, and toxicity effects of exosomes are evaluated using a zebrafish model.

 

8.Mouse Model Based Pharmacological Evaluation

The whitening, repair, anti-inflammatory, and toxicity effects of exosomes are evaluated using a mouse model.