Small Extracellular Vesicles/Exosome Particle Size Analysis Service

Exsome particle size detection is an important step in exosome research, helping researchers understand the size distribution, quantity distribution and purity of exosomes, which is vital for subsequent functional studies and further analysis. Particle size analysis provides an overall assessment of the distribution of exosome sizes, offering advantages such as accuracy, speed, and good reproducibility. It serves as an excellent complement to existing methods for exosome identification.

 

At NextGen Exsome, we use Flow NanoAnalyzer and ZetaView to characterize exosomes:

 

Nano Flow Cytometer (NanoFCM)

The Flow NanoAnalyzer overcomes the limitations of traditional flow cytometer in detecting particle size below 200nm. It encompasses the characterization of natural biological nanoparticles, including nanoparticles, sub-cellular structures, bacteria, viruses, and extracellular vesicles (exosomes), thereby opening a window to the nanoworld for flow cytometric analysis. By offering high-resolution, highly selective, and high-throughput detection of individual nanoparticles (ranging from 7 to 1000nm) in terms of their size and distribution, particle concentration, as well as biochemical properties, the Flow NanoAnalyzer provides a powerful tool for characterizing aspects critical to life sciences, biomedical research, and the development of nanotechnology.

 

Nanoparticle Tracking Analysis (NTA)

The ZetaView features single-particle tracking technology, which, combined with classical microelectrophoresis techniques and Brownian motion, has become a modern method of analysis. It’s automated calibration and focus functions provide a user-friendly and intuitive experience. By scanning the subvolume, zeta potential and particle size histograms from thousands of particles can be calculated. In addition, particle concentration can also be obtained through video counting analysis. The translational diffusion constant can be calculated by directly observing the Brownian motion of the particle to be measured. By testing the electrophoretic mobility, the zeta potential can be obtained.

 

Services at NextGen Exsome

1.Concentration and Particle Size of Exosomes or Extracellular Vesicles (NanoFCM)

Choose different standards according to different particle sizes to detect the concentration and particle size of exosomes or large vesicles.

 

Deliverables:

 

1)Sample particle size and concentration report in PDF format

2)Sample particle size distribution in XLSX format

3)Standard concentration report in PDF format

 

2.Concentration and Particle Size of Exosomes or Extracellular Vesicles (Zetaview)

Detect the concentration and size of exosomes or large vesicles.

 

Deliverables:

 

1)Sample particle size and concentration report in PDF format

2)Scatter plot in PNG format

3)Particle size distribution in TXT format

 

3.Zeta Potential of Exosomes or Extracellular Vesicles (Zetaview)

Detect the potential of exosomes or large vesicles.

 

Deliverables:

 

1)Sample report in PDF format

2)Potential data in TXT format

3)Particle motion trajectory in AVI format

4)Particle data in FCS format