Small Extracellular Vesicles/Exosomes Genomics

DNA within exosomes serves as a crucial biomarker, offering researchers insights into mechanisms of tumorigenesis, disease prognosis, and therapeutic outcomes. Sequencing DNA from exosomes involves acquiring DNA sequence data to facilitate analysis and research. The application of exosome DNA sequencing has extended to studies in cancer, neurodegenerative disorders, cardiovascular diseases, among others. Typically, exosome DNA sequencing projects encompass DNA extraction from exosomes, sequencing, and data analysis, allowing researchers to gain a comprehensive understanding of exosome DNA. This plays a vital role in providing a scientific foundation for disease diagnosis, management, and prognosis assessment.

 

Whole Genome Sequencing

Whole Genome Sequencing (WGS) leverages high-throughput sequencing technology to sequence the complete genomes of individuals or populations, identifying sequence or structural variations. For species with known genomes, sequencing various individuals or tissues reveals inter-individual or tissue-specific differences at the genomic level. This approach facilitates the discovery of a plethora of variation information, including Single Nucleotide Variants (SNVs), Insertions and Deletions (Indels), Structural Variations (SVs), and Copy Number Variations (CNVs). It is instrumental in identifying genes and mutations linked to diseases, elucidating disease mechanisms, uncovering genetic underpinnings, and understanding population genetics.

 

1.Process:

 

1)End-polishing (25°C, 30 min; 65°C, 15 min)

2)Ultra low input connection (25°C, 10 min)

3)Cleanup

4)Library amplification

 

Targeted Sequencing

Liquid phase chip capture sequencing offers a targeted genomic region analysis solution. It utilizes a multifactorial algorithm to design probes for specific genomic regions, followed by liquid-phase hybridization with genomic DNA to capture and enrich target sequences. Subsequent high-throughput sequencing on platforms like Illumina and Life Technologies facilitates the study of target regions across numerous samples, aiding in the discovery and validation of disease-associated genes and critical loci. This method has broad applications in clinical diagnostics, drug development, and agricultural research.

 

Services at NextGen Exsome

1.DNA Extraction

Using exosome-specific micro DNA extraction kit to extract DNA and detect the concentration.

 

Deliverables:

 

DNA concentration data in XLSX format

 

2.DNA Quality Control

Using capillary electrophoresis to detect the fragment distribution of nucleic acids.

 

Instrument stand:

 

Qsep100

 

Deliverables:

 

1)Sample analysis report in PDF format

2)Instrument raw data in BOPX format

 

3.Whole Genome Sequencing

Using a low-input DNA library preparation kit for whole-genome library construction and sequencing.

 

Deliverables:

 

1)Standard analysis report in PDF format

2)Sequencing raw data in 30G FASTQ format

 

4.Targeted Sequencing

Using a low-input DNA library preparation kit for targeted capture library construction and sequencing.

 

Deliverables:

 

1)Standard analysis report in PDF format

2)Sequencing raw data in 400M FASTQ format