Small Extracellular Vesicles/Exosomes Metabolomics
Exosome metabolomics research refers to obtaining comprehensive information on the metabolic status of exosomes through the measurement and analysis of metabolites in exosomes, thereby exploring the role and mechanism of exosomes in biological metabolism. Exosome metabolomics research can provide researchers with information on exosome metabolic regulation, disease occurrence and development, and treatment strategies. Exosome metabolomics technical service projects usually include the extraction of exosomes, the isolation and purification of metabolites, and the analysis of metabolites. Through these links, researchers can obtain comprehensive information on the metabolites of exosomes, including the types, contents, metabolic pathways, etc. of metabolites, and then analyze and study the metabolic regulation of exosomes. The metabolome describes the entire body of endogenous metabolic substances in an organism, which can be divided into non-targeted metabolome and targeted metabolome.
Non-targeted Metabolomics
Exosome non-targeted metabolomics is a technique based on orbitrap ultra-high resolution mass spectrometry, designed for the specific sample type of exosomes, capable of achieving the accurate identification of metabolites in a non-targeted metabolomics analysis. Generally, the metabolites in the sample are analyzed simultaneously qualitatively and quantitatively based on LC-MS technology, and the primary and secondary information are matched with the local self-built library and commercial standard database at the same time for substance identification. This method is suitable for detecting most metabolites, the technology is mature and stable, and applicable to various complex biological samples, making it a mainstream technology for studying metabolic changes.
Targeted Metabolomics
Targeted metabolomics is the detection and analysis of a specified list of metabolites, specifically targeting the metabolites of one or several pathways. The analysis emphasizes the use of a large number of natural and biological variant samples to validate pre-identified metabolites or identified potential biomarkers. It requires the use of standards for accurate qualitative and quantitative analysis. High-throughput targeted metabolomics can simultaneously achieve the detection of multiple types of substances in various sample types, obtaining precise qualitative and quantitative results without the need for re-validation. It is especially suitable for discovery metabolomics, clinical translation and industrialization research.
Services at NextGen Exsome
1.Non-targeted Exosome Metabolomics
Using non-target metabolomics methods for detection and standard data analysis, searches are conducted through a locally constructed database of 30,000 substances, which can provide qualitative and quantitative data and secondary qualitative mirror image assessments.
Deliverables:
1)A report in PDF format
2)The raw data of machine in WIFF format
2.Targeted Exosome Metabolomics
Using targeted metabolomics method for detection and standard data analysis, including energy metabolism, amino acids, bile acids, tryptophan (23 types), arachidonic acid, neurotransmitters, flavonoids, etc .
Deliverables:
1)A report in PDF format
2)The raw data of machine in WIFF format