Drug Loading Service for Exosome-Based Drug Delivery
Exosome-based drug delivery involve altering the surface molecular structure of exosomes or integrating novel biomolecular entities within them, with the aim of enhancing their capabilities in targeted delivery and therapeutic precision. This advanced technique provides researchers with enhanced strategies for exosomal-based treatments. Exosome-based drug delivery services typically involve several stages, including the extraction of exosomes, their modification or transformation, purification, and subsequent analysis. Through these meticulous procedures, researchers can obtain specifically modified or transformed exosomes, which are then subjected to stringent purification and comprehensive evaluation, ultimately facilitating their effective application in targeted delivery and therapeutic interventions.
Services at NextGen Exsome
1.Drug Loading Techniques
1)Sonication-mediated Exosome Cargo Loading
Ultrasonic treatment of exosomes using a probe sonicator enhances membrane permeability, allowing small molecular substances to diffuse into the exosomes.
Deliverables:
Cargo-Loaded exosomes and testing report
2)Reagent-mediated Exosome Cargo Loading
The use of cationic substances to promote interaction with lipid membranes and subsequent internalization effectively loads nucleic acids into the interior of exosomes.
Deliverables:
Cargo-Loaded exosomes and testing report
3)Electroporation-mediated Exosome Cargo Loading
Applying an electric field to form temporary hydrophilic pores on the exosome membrane, increasing its permeability and allowing small molecular substances to pass through these pores into the exosome.
Deliverables:
Cargo-Loaded exosomes and testing report
4)Genetically Engineered Exosome Cargo Loading
Utilizing the CP05 peptide conjugate’s ability to specifically bind to CD63, targeting molecules or functional molecules can be coupled to the exosome surface for targeted or functional purposes.
Deliverables:
Cargo-Loaded exosomes and testing report
2.Drug Loading Efficiency Testing
1)NanoFCM
Nano-Flow Cytometry (NanoFCM) is employed to assess drug loading efficiency by detecting the positivity rate of membrane surface proteins or nucleic acids (FITC-labeled).
Deliverables:
• Sample report in PDF format
• Original instrument data in FCS format
2)HPLC
Exosome drug loading efficiency is measured using High-Performance Liquid Chromatography (HPLC) methods.
Deliverables:
• Sample report in DOCX format
• Original instrument data in PDF format
3)LC-MS
Using Liquid Chromatography-Mass Spectrometry (LC-MS) to evaluate the drug loading efficiency of exosomes.
Deliverables:
• Sample report in DOCX format
• Original instrument data in PDF format
3.Cellular Modification
1) Lentivirus Overexpression
After transfecting plasmids and transducing viruses, the parent cells are made to increase the expression of target nucleic acids or proteins.
Deliverables:
• Exosomes
• Stable Cell Lines
• Viruses
• Parameter testing report
2)Lentivirus Mediate Interference
After transfecting plasmids and transducing viruses, the parent cells are made to decrease the expression of target nucleic acids or proteins.
Deliverables:
• Exosomes
• Stable Cell Lines
• Viruses
• Parameter testing report